Mitochondrial Aspartate Aminotransferase is a normal Constituent of human Serum
نویسندگان
چکیده
منابع مشابه
Detection and assay of mitochondrial aspartate aminotransferase in serum.
Aspartate aminotransfcrase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2. 6.1.1) comprises two isoen&ymcs. One is anionic, is associated with the soluble fraction of the cell and ordinarily forms the bulk of the scrum activity. The other is cationic, and is associated with the mitochondria (1—7). Each may in turn be subdivisible (8—10), but it is unlikely that this will influence the pres...
متن کاملAn immunochemical procedure for determination of mitochondrial aspartate aminotransferase in human serum.
An immunochemical procedure is described for quantitation of mitochondrial aspartate aminotransferase (m-AspAT; EC 2.6.1.1) activity in human serum specimens. Antibodies directed against purified soluble aspartate aminotransferase (s-AspAT) from human erythrocytes were produced in rabbits and partly purified. Antibody sufficient for analyses of > 6000 specimens could be obtained from 15 mL of r...
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We describe the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. We partly purified the antisera by selective column chromatography. T...
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The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AS...
متن کاملSerum levels of the mitochondrial isoenzym of aspartate aminotransferase in myocardial infarction and muscular dystrophy.
Aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1, glutamic-oxalacetic transaminase, GOT) comprises two isoenzymes. One is anionic and is associated principally with the soluble fraction of the cytoplasm, the other is cationic and is associated with the mitochondria [4]. A sensitive semi quantitative assay has been developed for cationic aspartate aminotransfe...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1970
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1970.8.6.611